Nucleolar localization of succinic dehydrogenase in human malignant cells with MTT
نویسندگان
چکیده
منابع مشابه
Studies on Succinic Dehydrogenase
About 2 years ago the author reported that the activity of purified preparations of succinic dehydrogenase is enhanced by phosphate buffer (2). This enhancement was dependent upon the phosphate concentration and appeared to be relatively specific for phosphate, since other buffers, such as Tris,l imidazole, histidine, glycylglycine, and arsenate did not induce it (2, 3). Arsenate, in fact, inte...
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The enzyme succinic semialdehyde dehydrogenase from pig brain has been 2000-fold purified by a combination of DEAE-cellulose, hydroxyapatite, and AMPSepharose chromatography. This preparation has a molecular weight of 160,000 and a specific activity of 5.3 pmol/min*mg at 25°C. The inhibition of succinic semialdehyde dehydrogenase by carbonyl compounds, i.e. P-pyridoxal and o-phthalaldehyde was ...
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During the investigation of the Krebs cycle in Azotobacter agile, strain 4.4 (Repaske and Wilson, 1953), it was found that whole cells normally required 20 minutes before adaptation to succinate, but succinoxidase activity could be demonstrated in cell-free extracts made from unadapted cells. If extracts prepared in a 10 kc Raytheon sonic oscillator were centrifuged at 145,000 G for 30 minutes,...
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Intracellular lactate dehydrogenase (LD) isoenzyme patterns were studied in the malignant cells of patients with a variety of lymphoid malignancies. These were compared with intracellular LD isoenzyme patterns of normal lymphoid cells and were also correlated with immunologic cell surface marker characteristics. Results showed that, in general, the malignant B cells of Burkitt's lymphoma and th...
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Although the inhibition of succinic dehydrogenase by oxalacetate is a generally accepted fact, there is a paucity of data on the subject. In 1937 Das (l), using a modified Thunberg technique, reported that the enzyme was 50 per cent inhibited by 2 X 1O-5 M oxalacetate when the succinate concentration was 0.025 M. In 1939 Potter (2) reported that the oxidation of succinate by liver and kidney ho...
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ژورنال
عنوان ژورنال: Experientia
سال: 1962
ISSN: 0014-4754,1420-9071
DOI: 10.1007/bf02172179